The ultrastructure of rat plasma lipoproteins
Identifieur interne : 000D83 ( Main/Exploration ); précédent : 000D82; suivant : 000D84The ultrastructure of rat plasma lipoproteins
Auteurs : I. Pasquali-Ronchetti [Italie] ; S. Calandra [Italie] ; M. Baccarani-Contri [Italie] ; M. Montaguti [Italie]Source :
- Journal of Ultrastructure Research [ 0022-5320 ] ; 1974.
Abstract
The ultrastructure of rat plasma lipoproteins has been studied by both the conventional negative staining technique and a new negative staining carbon-film technique that improves contrast, resolution, and preservation of biological material. Plasma lipoproteins were separated by preparative ultracentrifugation at densities of 1.006, 1.019, 1.063, 1.125, and 1.210 g/ml. By the carbon-film technique it is possible to have a detailed image of the surface of chylomicrons, which appear as large spherical particles completely covered by particles having either a spherical or a vesicular shape. Very low density lipoproteins, in contrast to the previously published observations, consist of two kinds of particles. One is spherical and has a smooth regular surface; the other is smaller, irregular in shape, and appears as an empty vesicle. Low density lipoproteins [LDL2 (1.019–1.063 g/ml)] were homogeneous both in size and shape (mean diameter, 20.0 nm). A close analysis of LDL2 surface revealed that LDL2 are composed of subunits 2.5–3.0 nm large that exclude the negative stain. Although these subunits appear to be arranged in a regular network, no geometrical symmetry is clearly detectable. High density lipoproteins [HDL2 (1.063–1.125 g/ml)] are very homogeneous spherical particles (mean diameter, 13.6 nm) and they tend to pack into large complexes and appear to be composed of subunits. Each subunit consists of an electron-dense area (2.0 nm wide) surrounded by an electron-transparent ring (ring-like structure). HDL3 are heterogeneous in both size and shape (5.0–40.0 nm). Ring-like structures are also present in HDL3 either as isolated particles or as constituents of large aggregates. The results are discussed with regard to the recent views on the interpretation of electron microscope images of negatively stained biological material.
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DOI: 10.1016/S0022-5320(75)80134-1
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Plasma lipoproteins were separated by preparative ultracentrifugation at densities of 1.006, 1.019, 1.063, 1.125, and 1.210 g/ml. By the carbon-film technique it is possible to have a detailed image of the surface of chylomicrons, which appear as large spherical particles completely covered by particles having either a spherical or a vesicular shape. Very low density lipoproteins, in contrast to the previously published observations, consist of two kinds of particles. One is spherical and has a smooth regular surface; the other is smaller, irregular in shape, and appears as an empty vesicle. Low density lipoproteins [LDL2 (1.019–1.063 g/ml)] were homogeneous both in size and shape (mean diameter, 20.0 nm). A close analysis of LDL2 surface revealed that LDL2 are composed of subunits 2.5–3.0 nm large that exclude the negative stain. Although these subunits appear to be arranged in a regular network, no geometrical symmetry is clearly detectable. High density lipoproteins [HDL2 (1.063–1.125 g/ml)] are very homogeneous spherical particles (mean diameter, 13.6 nm) and they tend to pack into large complexes and appear to be composed of subunits. Each subunit consists of an electron-dense area (2.0 nm wide) surrounded by an electron-transparent ring (ring-like structure). HDL3 are heterogeneous in both size and shape (5.0–40.0 nm). Ring-like structures are also present in HDL3 either as isolated particles or as constituents of large aggregates. The results are discussed with regard to the recent views on the interpretation of electron microscope images of negatively stained biological material.</div></front></TEI><affiliations><list><country><li>Italie</li></country></list><tree><country name="Italie"><noRegion><name sortKey="Pasquali Ronchetti, I" sort="Pasquali Ronchetti, I" uniqKey="Pasquali Ronchetti I" first="I." last="Pasquali-Ronchetti">I. Pasquali-Ronchetti</name></noRegion><name sortKey="Baccarani Contri, M" sort="Baccarani Contri, M" uniqKey="Baccarani Contri M" first="M." last="Baccarani-Contri">M. Baccarani-Contri</name><name sortKey="Calandra, S" sort="Calandra, S" uniqKey="Calandra S" first="S." last="Calandra">S. Calandra</name><name sortKey="Montaguti, M" sort="Montaguti, M" uniqKey="Montaguti M" first="M." last="Montaguti">M. Montaguti</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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